MC-Transaction on Biotechnology, 2011, Vol. 3, No. 1, e2
Quantitative Analysis and Mass-Spectra Explanation for Diterpenoid Glycoside Atractyloside (ATR) in Traditional Chinese Herbs
Liang-Yu Chen1 *、Chi-Xiang Chen2、Chin-Kuo Lee3
1. Department Biotechnology, School of Health Technology, Ming-Chuan University, (Taoyuan, Taiwan, R.O.C.)
2. King-Car Food Industrial Corp., (Chung-Li, Taiwan, R.O.C.)
3. Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, (Taipei, Taiwan, R.O.C.)
Corresponding author: Liang-Yu Chen [ loknath@mail.mcu.edu.tw ]
Received 2 March 2011/Accepted 2 April 2011/Online published 3 April 2011
Abstract
Today, the ethnopharmacology is popular to treat disease, the herbs were used as considerable medicinal ingredients, but also the case with toxic substances is often due to improper poisoning, such as taking aristolochic acid caused kidney failure. Toxic risk evaluation of these plants in physiology is a very important issue to be facilitated by the reliable analytical method. Atractyloside (ATR) is one of a group of diterpenoid glycosides. The interest for these compounds was stimulated by the high toxicity of both glucosides, responsible of many deadly poisoning in past time. ATR occurs naturally in many Asteraceae plants, which are used as herbal medicines in Orient. The dried root of A. macrocephala and A. lancea are important crude drugs used in herbal medicine. ATR components were acidized and extracted from samples, then after the TMSI and pyridine derivatives for GC-MSD analysis. The ionic fragments in mass spectra were identified for TMS derivatives of ATR. The quantitative results showed that the ATR contents of 8980 ppm and 9230 ppm were detected in A. macrocephala and A. lancea, respectively. Others, the rare ATR was found in coffee beans for comparing.
Keyword: Atractylodes macrocephala, Atractylodes lancea, atractyloside, GC/MSD
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