MC-Transaction on Biotechnology, 2012, Vol. 4, No. 1, e5
Effects of Clinacanthus nutans (Burm.f) Lindau leaf extracts on protection of plasmid DNA from riboflavin photoreaction
Jeu-Ming P. Yuann, Jr-Shiuan Wang, Hong-Lin Jian, Chin-Chang Lin, Ji-Yuan Liang*
Department of Biotechnology, School of Health Technology, Ming Chuan University, (Taoyuan, Taiwan, R.O.C.)
Corresponding author: Ji-Yuan Liang [liang121@mail.mcu.edu.tw]
Received 25 August 2012/Revised 10 September 2012/Accepted 12 September 2012/Online published 13 September 2012
Abstract
Clinacanthus nutans (Burm.f) (CN) Lindau is widely grown in tropical Asia and is an important herbal medicine in China, Malaysia and Thailand. This study inspects the antioxidant activity and protective effects of Clinacanthus nutans extracts on plasmid DNA integrity in E. coli. The antioxidant activities of Clinacanthus nutans are lower compared with green tea. The superoxide dismutase (SOD) activity and total phenolic contents of green tea are almost 30- and 10-fold higher than that of Clinacanthus nutans leaves, respectively. However, Clinacanthus nutans leaf extract’s retention of the integrity of super-coiled plasmid DNA under riboflavin photochemical treatment was shown to be better in comparison with extracts of green tea.
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