李御賢 (Lee, Yun-Shien) Professor 教授
長庚醫院兼任老師
Ph.D., National Defense Medical Center
Specialization: 基因微陣列分析、基因體學 (Microarray, Genomics)
E-mail: bojack@mail.mcu.edu.tw
PSTReader : Program for Reading Parent-Sibling Tracing
A、發表著作
2009
1. Lee YS*, Chen CH; Tsai CN, Tsai CL, Chao A, Wang TH. (2009) Microarray Labeling Extension Values: Laboratory Signatures for Affymetrix GeneChips. Nucleic Acids Research 2009 May; 37 (8) (Impact factors: 6.954) *First Author
2. Chao A, Tsai CL, Wei PC, Hsueh S, Chao AS, Wang CJ, Tsai CN, Lee YS*, Wang TH*, Laia CH. (2009) Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma. Cancer Letters (Accepted) (Impact factor: 3.398). *correspondence author
3. Soong YK, Wang TH, Lee YS, Chen CP, Chang CL, Ho SY, Chao AS, Cheng PJ, Chang SD. (2009) Genome-wide Detection of Uniparental Disomy in a Fetus With Intrauterine Growth Restriction Using Genotyping Microarrays. Taiwan J Obstet Gynecol. 2009 Jun;48(2):152-8.
4. Huang CC, Lee TJ, Chang PH, Lee YS, Chuang CC, Jhang YJ, Chen JW, Chen CW, Tsai CN. (2009) Desmoglein 3 is overexpressed in inverted papilloma and squamous cell carcinoma of sinonasal cavity. The Laryngoscope (Accepted) (Impact factor: 1.801).
5. Yang SW, Lee YS, Chen TA, Wu CJ, Tsai CN. (2009) Human papillomavirus in oral leukoplakia is no prognostic indicator of malignant transformation. Cancer Detect. Prev. (Accepted) (Impact factor: 1.600)
2008
6. Lee YS*, Chao A, Chao AS, Chang SD, Chen CH, Wu WM, Wang TH, Wang HS. (2008) CGcgh: A tool for molecular karyotyping using DNA microarray-based comparative genomic hybridization (array-CGH). Journal of Biomedical Science. 2008 Nov;15(6):687-96. Epub 2008 Aug 19. (Impact factor: 2.024). *First Author
7. Lee YS*, Tsai CN, Tsai CL, Chang SD, Hsueh DW, Liu JT, Ma CC, Lin SH, Wang TH, Wang HS: (2008) Comparison of whole genome amplification methods for further quantitative analysis with microarray-based comparative genomic hybridization. Taiwanese Journal of Obstetrics & Gynecology. 2008 Mar;47(1):32-41.(non-SCI) *First Author
8. Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS. Macrophage Inflammatory Protein-3{alpha} Is a Novel Serum Marker for Nasopharyngeal Carcinoma Detection and Prediction of Treatment Outcomes. Clin Cancer Res. 2008 Nov 1;14(21):6979-87. (Impact factor: 6.25)
9. Shiue HS, Lee YS, Tsai1 CN, Hsueh YM, Sheu JR, Chang HH. (2008) DNA Microarray Analysis of the Effect on Inflammation in Patients Treated with Acupuncture for Allergic Rhinitis. Journal of Alternative and Complementary Medicine 2008, 14(6): 689-698 (Impact factor: 1.526).
10. Yang SW, Lee MH, Lee YS, Huang SH, Chen TA, Fang TJ. (2008) Analysis of Life-Threatening Complications of Deep Neck Abscess and the Impact of Empiric Antibiotics. ORL J Otorhinolaryngol Relat Spec. 2008 May 16;70(4):249-256. (Impact factor: 0.704)
11. Tien YJ, Lee YS, Wu HM, and Chen CH. (2008) Methods for Simultaneously Identifying Coherent Local Clusters with Smooth Global Patterns in Gene Expression Profiles. BMC Bioinformatics 2008, 9:155 doi:10.1186/1471-2105-9-155 (Impact factor: 3.617)
12. Chen TB, Lu HHS, Lee YS, Lan HJ: (2008) Segmentation of cDNA Microarray Images by Kernel Density Estimation. Journal of Biomedical Informatics 2008 Dec;41(6):1021-7. Epub 2008 Mar 7. doi:10.1016/j.jbi.2008.02.007 (Impact factor: 2)
13. Chao A, Wang, TH, Lee YS, Hong JH, Tsai CN, Chen CK, Tsai CS, Chao AS and Lai CH. Analysis of functional groups of differentially expressed genes in the peripheral blood of patients with cervical cancer undergoing concurrent chemoradiation treatment. Radiation Research. 2008 Jan;169(1):76-86. (Impact factor: 2.602, 2006, 21/85=0.24radiology, nuclear medicine, medical image)
14. Hsieh SY, Tseng CL, Lee YS, Kuo AJ, Sun CF, Lin YH, Chen JK: Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS. Mol Cell Proteomics. 2008 Feb;7(2):448-56. Epub 2007 Nov 27. (Impact factor: 9.62)
15. Chang KP, Hao SP, Tsang NM, Chang YL, Cheng MH, Liu CT, Lee, YS, Tsai CL, Lee TJ, Wang TH, Tsai CN. Promoter Polymorphisms and gene expression of DNMT3B and the Risk of Nasopharyngeal Carcinoma for Taiwanese people: A case-control study Oncology Reports Oncol Rep. 2008 Jan;19(1):217-22 (Impact factor: 1.567)
2007
16. Tsai, MS, Hwang, SM, Chen, KD, Lee, YS, Hsu, LY, Chang YJ, Wang CN, Peng HH, Chang YL, Chao AS, Chang SD, Lee KD, Wang TH, Wang HS, Soong YK. (2007) Functional Network Analysis on the Transcriptomes of Mesenchymal Stem Cells Derived from Amniotic Fluid, Amniotic Membrane, Cord Blood, and Bone Marrow. Stem Cells, Published online Jun 7, 2007; DOI:10.1634/ stemcells.2007-0023 (Impact factor: 7.924)
17. Chen, SY, Chang, YC, Lee, YS, Chao, SC, Tsao, KC, Lin, TY, Ko, TI, Tsai, CN, Chiu, CH (2007) Molecular Epidemiology and Clinical Manifestations of Viral Gastroenteritis in Hospitalized Pediatric Patients in Northern Taiwan. Journal of Clinical Microbiology. (doi:10.1128/JCM.01519-06, 18 April 2007) 45(6):2054–2057 (Impact factor: 3.445, 21/89=0.24, microbiology, )
18. Chang, KP, Hao, SP, Liu, CT, Cheng, MH, Chang, YL, Lee, YS, Wang, TH and Tsai, CN (2007). "Promoter polymorphisms of DNMT3B and the risk of head and neck squamous cell carcinoma in Taiwan: A case-control study." Oral Oncol. 2007 Apr;43(4):345-51 (Impact factor: 2.266)
2006
19. Chao, A, Lee, YS, Chao, AS, Wang, TH and Chang, SD (2006). Microarray- based Comparative Genomic Hybridization Analysis of Wolf-Hirschhorn Syndrome in a Fetus with Deletion of 4p15.3 to 4pter. Birth Defects Research Part A: Clinical and Molecular Teratology. 2006 Oct;76(10):739-43 (Impact factor: 2.005, 33/76=0.43, toxicology)
20. Chao, A, Wang, TH, Lee, YS, Hsueh, S, Chao, AS, Chang, TC, Kung, WH, Huang, SL, Chao, FY, Wei, ML and Lai, CH (2006). "Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression." Int J Cancer 119(1): 91-8. (Impact factor: 4.700)
21. Pan, YS, Lee, YS, Lee, YL, Lee, WC and Hsieh, SY (2006). "Differentially profiling the low-expression transcriptomes of human hepatoma using a novel SSH/microarray approach."BMC Genomics 7: 131. (Impact factor: 4.092)
22. Sun, CC, Su Pang, JH, Cheng, CY, Cheng, HF, Lee, YS, Ku, WC, Hsiao, CH, Chen, JK and Yang, CM (2006). "Interleukin-1 Receptor Antagonist (IL-1RA) Prevents Apoptosis in ex vivo Expansion of Human Limbal Epithelial Cells Cultivated on Human Amniotic Membrane." Stem Cells. 2006;24;2130-2139. (Impact factor: 6.094)
23. Tien, YJ, Lee, YS, Wu HM. and Chen, CH. (2006) Integration of clustering and visualization methods for simultaneously identifying coherent local clusters with smooth global patterns in gene expression profiles. Technical Report 2006-11, Institute of Statistical Science, Academia, Taiwan. (non-SCI)
24. Wang, TH, Chan, YH, Chen, CW, Kung, WH, Lee, YS, Wang, ST, Chang, TC and Wang, HS (2006). "Paclitaxel (Taxol) upregulates expression of functional interleukin-6 in human ovarian cancer cells through multiple signaling pathways." Oncogene 25(35): 4857-66. (Impact factor: 6.872)
25. Wu, CM, Lee, YS, Wang, TH, Lee, LY, Kong, WH, Chen, ES, Wei, ML, Liang, Y and Hwang, TL (2006). "Identification of differential gene expression between intestinal and diffuse gastric cancer using cDNA microarray." Oncol Rep 15(1): 57-64. (Impact factor: 1.572)
2005
26. Lee, YS*, Chen, CH, Chao, A, Chen, ES, Wei, ML, Chen, LK, Yang, KD, Lin, MC, Wang, YH, Liu, JW, Eng, HL, Chiang, PC, Wu, TS, Tsao, KC, Huang, CG, Tien, YJ, Wang, TH and Wang, HS (2005). "Molecular signature of clinical severity in recovering patients with severe acute respiratory syndrome coronavirus (SARS-CoV)." BMC Genomics 6: 132. (Impact factor: 4.092) *First Author
27. Wu, WM, Tsai, HJ, Pang, JH, Wang, HS, Hong, HS and Lee, YS* (2005). "Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction." Anal Biochem 339(2): 290-6. (Impact factor: 2.670), *correspondence author
28. Wu, WM, Tsai, HJ, Pang, JH, Wang, TH, Wang, HS, Hong, HS and Lee, YS* (2005). "Linear allele-specific long-range amplification: a novel method of long-range molecular haplotyping." Human Mutation 26(4): 393-4. (Impact factor: 7.923), *correspondence author
2004
29. Shih, CH, Chen, SL, Yen, CC, Huang, YH, Chen, CD, Lee, YS and Lin, KH (2004). "Thyroid hormone receptor-dependent transcriptional regulation of fibrinogen and coagulation proteins." Endocrinology 145(6): 2804-14. (Impact factor: 5.313)
30. Wang, TH, Lee, YS, Chen, ES, Kong, WH, Chen, LK, Hsueh, DW, Wei, ML and Wang, HS (2004). "Establishment of cDNA microarray analysis at the Genomic Medicine Research Core Laboratory (GMRCL) of Chang Gung Memorial Hospital." Chang Gung Med J 27(4): 243-60. (non-SCI)
Before 2003
31. Lee, YS, Hsu, YH and Lin, NS (2000). "Generation of subgenomic RNA directed by a satellite RNA associated with bamboo mosaic potexvirus: analyses of potexvirus subgenomic RNA promoter." J Virol 74(22): 10341-8. (Impact factor: 5.178)
32. Hsu, YH, Lee, YS, Liu, JS and Lin, NS (1998). "Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants." Molecular Plant-Microbe Interactions 11: 1207-1213. (Impact factor: 3.928)
33. Lee, YS, Lin, BY, Hsu, YH, Chang, BY and Lin, NS (1998). "Subgenomic RNAs of bamboo mosaic potexvirus-V isolate are packaged into virions." J Gen Virol 79 ( Pt 7): 1825-32. (Impact factor: 3.013)
34. Lin, NS, Lee, YS, Lin, BY, Lee, CW and Hsu, YH (1996). "The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene." Proc Natl Acad Sci USA 93(7): 3138-42. (Impact factor: 10.231)
B 專利
1. Kung, Patrick C.; (Cambridge, MA) ; Peck, Konan; (Taipei, TW) ; Lee, Yun-Shien; (Keelung, TW) ; Sher, Yuh-Pyng; (Shijr Jen, TW) ; Cheng, Yung-Chi; (Woodbridge, CT), PHYTOMICS:A genomic-based approach to herbal composition. United States Patent Application 20030207270, (Nov 6, 2003)
C. 研討會論文或推廣教育課程
1. 李御賢 (2009) Microarray gene expression databases and Microarray gene expression analysis. 交通大學生物資訊所「系統生物平台應用與電腦輔助藥物設計」推廣教育碩士學分課程。(2009/7/10)
2. 李御賢 (2009) 基因晶片分析. 清華大學生醫工程與環境科學系「奈米生醫光電及其應用」推廣教育碩士學分班。(2009/7/23)
3. 李御賢 (2008) Haplotype Analysis of the Copy Number Variation in Cancer Genomic DNA.國立臺北大學97統計學術研討會, 國立臺北大學三峽校區 (2008/12/19)
4. 李御賢 (2008) Microarray gene expression databases and Microarray gene expression analysis. 交通大學生物資訊所「系統生物平台應用與電腦輔助藥物設計」推廣教育碩士學分課程。(2008/7)
5. 李御賢 (2008) CG-haplotype - program to analyze the copy number polymorphism in DNA haplotype. 長庚醫院小兒過敏科的Research Meeting, 宜蘭 (2008/08/29)
6. 李御賢 (2007) Multiple human tissue classification using RNA degradation profile by Affymetrix GeneChip. 台灣生物資訊與系統生物學研討會 Symposium of Bioinformatics and Systems Biology in Taiwan, 國立中正大學 (嘉義) (2007/9/4)
7. 李御賢 (2007) , Haplotyping the DNA amplification region in the cancer genome using Affymetrix 10K DNA Chip. Second WorkShop on System Biology and Bioinformatics,中央大學系統生物與生物資訊研究所主辦,(2007/4/14)
8. 李御賢 (2007) Microarray gene expression databases and Microarray gene expression analysis. 交通大學生物資訊所「系統生物平台應用與電腦輔助藥物設計」推廣教育碩士學分課程。(2007/7/31)
9. 李御賢 (2007) Microarray data analysis. 交通大學生物資訊所「系統生物學分析工具與資料庫之整合應用」推廣教育碩士學分課程。(2007/7/11)
10. 李御賢 (2006) Microarray gene expression databases and Microarray gene expression analysis. 交通大學生物資訊所「系統生物平台應用與電腦輔助藥物設計」推廣教育碩士學分課程。(2007/8/1)
11. 李御賢 (2006) Microarray data analysis. 交通大學生物資訊所「系統生物學分析工具與資料庫之整合應用」推廣教育碩士學分課程。(2006/7/12)
12. 李御賢 (2005) How do you conduct a microarray experiment? 2005 國立臺灣大學生物資訊研討會,生物資源暨農學院農業自動化教學及研究中心主辦。(2005/1/18)
13. Chen, C. H., Lu, H. S., Liao C. T., Chen, C. H., Yang, U. C., Lee, Y. S. (2003) Gene Expression Analysis Refining System (GEARS) via Statistical Approach: A Preliminary Report. The 14th International Conference on Genome Informatics,Yokohama, Japan (Abstract available in Genome Informatics 14: 316-317)
D. 近五年內曾參與之專題研究計畫
1. 利用生物資訊學研究SARS病人血液中RNA的基因表現圖譜 主持人
2004/8/1~2005/7/31 國科會 NSC 93-2320-B-130-001
2. 先天性乳糜胸的遺傳性研究 主持人
2005/8/1~2006/7/31 國科會 NSC 94-2320-B-130-002
3. 以Affymetrix DNA Chip 的RNA degradation profile 進行人類組織的分類 主持人2006/8/1~2007/7/31 國科會 NSC 95-2311-B-130-001
4. 利用生物資訊學與分子生物學分析癌細胞DNA數量增加或異質性流失之區域的單體型 主持人2007/8/1~2008/7/31 國科會 NSC 96-2320-B-130-001
5. 發展偵測與驗證單套體中DNA拷貝數多樣性的方法 主持人
2008/8/1~2010/7/31 國科會 NSC 97-2320-B-130-001-MY2
6. NSC 國家基因型計畫--進階生物資訊學核心設施
共同主持人 2005/01/05~2008/04/30 (Co-investigator)
7. NSC 國家基因型計畫--進階生物資訊學核心設施
共同主持人 2008/05/01~2011/04/30 (Co-investigator)
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